Berthold Bioanalityc Microplate and Elisa

Berthold Bioanalityc is a company engaged in laboratory equipment that provides laboratory equipment from simple to sophisticated. This German company already has a lot of products that can be purchased with quality and good quality assurance. Not only that, but you can also get goods with the appropriate warranty. Then what are the laboratory tools provided by this company? Some we will discuss here. You are also available to check the website berthold-bio.com to get more information about the products.

 

This is about Microplate Reader. The microplate reader functions the same as UV Vis. Chemical kids must know about UV Vis. UV Vis, in short, is a tool to find out the concentration based on the color of the sample, using visible light, as well as Ultra Violet light. How come you know concentration? Because if a colored sample is shot with UV or Visible light with a wavelength that matches the color of the sample, there will be a difference in absorbance of the sample based on the "thick" or "thin" color of the sample. For example, a slightly blue sample absorbance is smaller than a sample that is very blue when shot by light with a blue wavelength. Well, to know the right wavelength, the blue sky or dark blue means, in the tool you can find the maximum wavelength, if in the graph, x = wavelength, y = absorbance, choose the highest absorbance, y is the biggest.

 

Here, Berthold Bioanalityc also made the ELISA workstation. Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical technique that is mainly used in the field of immunology to detect the presence of antibodies or antigens in a sample. ELISA has been used as a diagnostic tool in the medical field, plant pathology, as well as various industrial fields. In a simple sense, a number of unknown antigens are affixed to a surface, then specific antibodies are washed on the surface so that they will bind to the antigen. These antibodies are bound to an enzyme, and at the last stage, a substance that can be changed by the enzyme is added to a signal that can be detected. In fluorescence ELISA, when light with a certain wavelength is illuminated in a sample, the antigen/antibody complex will fluoresce so that the number of antigens in the sample can be inferred based on the magnitude of fluorescence.

 

The use of ELISA involves at least one antibody with specificity for a particular antigen. Samples with an unknown number of antigens are immobilized on a solid surface (usually a polystyrene microtiter plate), either non-specific (through surface absorption) or specific (through capture by other antibodies specific to the same antigen, called 'sandwich') ELISA). After the antigen is immobilized, detecting antibodies are added, forming a complex with an antigen. Detecting antibodies can also bind to enzymes, or can be detected directly by secondary antibodies that bind to enzymes through bioconjugation. Between each stage, the plate must be washed with a gentle detergent solution to remove excess protein or unbound antibodies. There are still many tools you can get in Berthold Bioanalityc.

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